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« เมื่อ: ตุลาคม 28, 2006, 10:13:36 AM »




1. The whitespot proteins VP19 and VP28 have been replicated in bacteria and used to vaccinate Penaeus monodon (injection, oral routes and immersion). Purified dsRNA and siRNA molecules were used as the vaccine agent. They induced a sequence-independent, antiviral immunity when injected in shrimp.

2. The efficacy of vaccines made from inactivated WSSV (whitespot syndrome virus, with and without immunostimulants or killed Vibrio penaeicida) and recombinant proteins of WSSV (VP26 & VP28) have been tested by intramuscular vaccination in kuruma shrimp, P. japonicus.

3. WSSV proteins VP28 and VP19 were replicated in silkworms using a baculovirus (HyNPV) expression system and fed to crayfish with significant effects.

4. Some work has also been reported on the development of vaccines against WSSV with peptidoglycan, lipopolysaccharide and glucan.

5. Three structural proteins of WSSV (VP36A, VP36B and VP31) have been isolated and used in vaccines with significant results.

6. VP19-antiserum and anti r-VP28 have been used to neutralize WSSV.

7. VP19 and VP28 proteins have been used as oral, immersion and injected vaccines against WSSV in crawfish (Procambarus clarkii). Vaccine-coated food pellets were fed to the crawfish for 25 days and then the crawfish were challenged with the muscle of WSSV-infected crayfish at a rate of two grams per individual for two days. The cumulative mortality was monitored for 25 days. Survival in the group given VP28 protein was 86%. The immersion group had survivals of 63%; the injected group, 91%.

8. The methanolic extracts of five different herbal medicinal plants fed orally as immunostimulants against WSSV in P. monodon resulted in survivals of 60%.

9. AntiTrVP28:19 egg yolk antibodies have been tested and found to neutralize shrimp whitespot in P. chinensis.

Source: AquaCulture Asia Pacific (Editor/Publisher, Zuridah Merican, email zuridah@aquaasiapac.com, webpage www.aquaasiapac.com). Developing Vaccines Against WSSV. Rajeev Kumar Jha (jha_fish@yahoo.co.in), Zi Rong Xu and Maddur Lingappa Umashankara. Volume 2, Number 5, Page 16, September/October 2006
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